Journal: Chinese Medicine
Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
doi: 10.1186/s13020-025-01203-0
Figure Lengend Snippet: Pulmonary Protective Effects of SMEVs in LPS-induced mouse Model. A Schematic illustration of the experimental protocol. B Body weight variation profile (n = 6, x̅ ± s). C Last Body Mass Index (n = 6, x̅ ± s). D Lung index of mice in each group (n = 6, x̅ ± s). E mRNA expression levels of IL-1β, IL-6 and TNF-α in lung tissues determined by RT-qPCR (n = 6, x̅ ± s). F Representative H&E-stained lung tissue sections. G Immunofluorescence analysis of the CD31 (red) and VE-cadherin (green) expression in lung tissues
Article Snippet: The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.).
Techniques: Expressing, Quantitative RT-PCR, Staining, Immunofluorescence
